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PD-1 PD-L1 IgG2a CD3ε CD28 Ly6G IFNγ IL-4
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询价

RecombiMAb anti-mouse PD-1 (CD279)

货号 #CP094
克隆号:
RMP1-14-CP094
反应种属:
Mouse

￥5,824 - ￥154,539

选择参数
  • 100 mg - ￥154,539
  • 50 mg - ￥110,386
  • 25 mg - ￥78,848
  • 5 mg - ￥22,573
  • 1 mg - ￥5,824
  • 定制包装(请询价)
RecombiMAb anti-mouse PD-1 (CD279)
产品说明书 安全数据表

产品详情

The RMP1-14-CP094 monoclonal antibody is a recombinant, Fc-engineered chimeric version of the original RMP1-14 antibody. The variable domain sequences are identical but the constant region sequences have been switched from Rat IgG2a, κ to Mouse IgG2c, κ for use in murine models. Species-matched chimeric antibodies exhibit regulated effector functions—including Fc receptor binding and complement activation—and result in less immunogenicity and formation of anti-drug antibodies (ADAs) than xenogenic antibodies in animal models. Mouse IgG2c and IgG2a are allelic variants of the same isotype, differing by strain-specific genetics. Mouse strains such as C57Bl/6, C57Bl/10, SJL, and NOD mice possess the Igh1-b allele resulting in only the expression of IgG2c. However, mouse strains such as BALB/c and Swiss Webster mice possess the Igh1-a allele which results in only the expression of IgG2a. It is important to consider matching the Ig-haplotype of the receiving mice to the isotype of the injected antibody to avoid eliciting undesired immune responses and ensuring better antibody tolerance, pharmacokinetics, and efficacy in chronic in vivo models like PD-1 blockade. RMP1-14-CP094 has an effector function competent Fc domain allowing for activation of Fcγ receptors (FcγRs) to trigger antibody‑dependent cellular cytotoxicity (ADCC), antibody‑dependent cellular phagocytosis (ADCP), complement‑dependent cytotoxicity (CDC) and opsonization to promote target cell depletion. The mouse IgG2c isotype demonstrates strong effector functions due to potent interaction with mFcγRIV, which is functionally similar to the FcγRIIIa receptor involved in human ADCC. The highly controlled sequence and lack of genetic drift in recombinant antibodies provide more reliable and reproducible results over hybridoma derived antibodies. RMP1-14-CP094 reacts with mouse Programmed cell death protein 1 (PD-1, CD279) is a type I transmembrane immune checkpoint receptor of the immunoglobulin superfamily expressed primarily on activated T cells, B cells, and some NK cells, where it dampens T cell receptor signaling to maintain peripheral tolerance and prevent autoimmunity through ITIM/ITSM motifs in its cytoplasmic tail that recruit phosphatases and attenuate activation. Its ligands PD-L1 and PD-L2 are expressed on hematopoietic and many non-hematopoietic cells, including tumor cells, allowing cancers to exploit the PD-1 pathway to induce T cell exhaustion, reduce cytokine production, and evade immune destruction within the tumor microenvironment. RMP1-14-CP094 specifically binds an epitope on PD-1, inhibiting its interaction with ligands PD-L1 and PD-L2 to reinvigorate T cell proliferation, cytokine production (e.g., IFN-γ), and anti-tumor responses in syngeneic and genetically engineered mouse tumor models.

参数

同种型 Mouse IgG2c, κ
推荐的同型对照 InVivoMAb mouse IgG2c isotype control, anti-dengue virus
推荐稀释缓冲液 InVivoPure pH 7.0 Dilution Buffer
免疫原 Syrian Hamster BKH cells transfected with mouse PD-1 cDNA
Reported Applications in vivo blocking of PD-1/PD-L signaling* *Reported for the original rat IgG2a RMP1-14 antibody
形式 PBS, pH 7.0 Contains no stabilizers or preservatives
内毒素 ≤0.5EU/mg (≤0.0005EU/μg) Determined by LAL assay
聚合 <5% Determined by SEC
纯化 ≥95% Determined by SDS-PAGE
无菌性 0.2 μM filtration
生产 Purified from CHO cell supernatant in an animal-free facility
提纯 Protein A
RRID AB_2927529
分子量 150 kDa
小鼠病原体试验 Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Minute Virus: Negative Mouse Hepatitis Virus: Negative Reovirus Screen: Negative Lymphocytic Choriomeningitis virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Mouse Rotavirus: Negative Theiler's Murine Encephalomyelitis: Negative Ectromelia/Mousepox Virus: Negative Hantavirus: Negative Polyoma Virus: Negative Mouse Adenovirus: Negative Sendai Virus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Mouse Cytomegalovirus: Negative K Virus: Negative
保存 The antibody solution should be stored at the stock concentration at 4°C. Do not freeze.

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