RecombiMAb anti-mouse TNFα
货号 #CP074
克隆号:
XT3.11-CP074
反应种属:
Mouse
￥5,824 - ￥78,848
产品详情
The XT3.11-CP074 monoclonal antibody is a recombinant, chimeric version of the original XT3.11 antibody. The variable domain sequences are identical but the constant region sequences have been switched from rat IgG1 to murine IgG2a, kappa for use in murine models. Antibody-dependent cellular cytotoxicity (ADCC) is a contributing mechanism of action of anti-TNF antibodies, since anti-TNF antibodies have been confirmed to induce cytotoxic effects on TNF-producing cells via ADCC and complement-dependent cytotoxicity (CDC). Murine IgG2a mediates ADCC primarily through its high-affinity binding to the mFcγRIV receptor, a key effector mechanism in the mouse immune system for targeting diseased or infected cells. In contrast to other isotypes, mIgG2a demonstrates strong effector functions due to this potent interaction with mFcγRIV, which is functionally like the FcγRIIIa receptor involved in human ADCC. Species-matched chimeric antibodies result in less immunogenicity and formation of anti-drug antibodies (ADAs) than xenogenic antibodies in animal models. The highly controlled sequence and lack of genetic drift in recombinant antibodies provide more reliable and reproducible results over hybridoma derived antibodies. The XT3.11-CP074 monoclonal antibody reacts with mouse TNFα (tumor necrosis factor-alpha) a multifunctional proinflammatory cytokine. TNFα exists as a soluble 17 kDa monomer, which forms homotrimers in circulation or as a 26 kDa membrane-bound form. TNFα belongs to the TNF superfamily of cytokines and signals through its two receptors, TNFR1 and TNFR2 which can be activated by both the soluble trimeric and membrane-bound and forms of TNFα. TNFα is primarily produced by macrophages in response to foreign antigens such as bacteria (lipopolysaccharides), viruses, and parasites as well as mitogens and other cytokines but can also be expressed by monocytes, neutrophils, NK cells, CD4 T cells and some specialized dendritic cells. TNFα is known to play key roles in a wide spectrum of biological processes including immunoregulation, cell proliferation, differentiation, apoptosis, antitumor activity, inflammation, anorexia, cachexia, septic shock, hematopoiesis, and viral replication. TNFα dysregulation has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Mouse and human TNFα share 79% amino acid sequence identity however, mouse TNFα is glycosylated while human TNFα is not. TNFα knockout animals display defects in response to bacterial infection, characterized by defects in forming organized follicular dendritic cell networks and germinal centers with a lack of primary B cell follicles.参数
| 同种型 | Mouse IgG2a, κ |
|---|---|
| 推荐的同型对照 | RecombiMAb mouse IgG2a isotype control, unknown specificity |
| 推荐稀释缓冲液 | InVivoPure pH 7.0 Dilution Buffer |
| 免疫原 | Recombinant mouse TNFα |
| Reported Applications | Western blot in vivo TNFα neutralization* in vitro TNFα neutralization* *Reported for the original rat IgG1 XT3.11 antibody |
| 形式 | PBS, pH 7.0 Contains no stabilizers or preservatives |
| 内毒素 | ≤0.5EU/mg (≤0.0005EU/μg) Determined by LAL assay |
| 聚合 | <5% Determined by SEC |
| 纯化 | ≥95% Determined by SDS-PAGE |
| 无菌性 | 0.2 μM filtration |
| 生产 | Purified from mammalian cell supernatant in an animal-free facility |
| 提纯 | Protein G |
| 分子量 | 150 kDa |
| 小鼠病原体试验 | Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Minute Virus: Negative Mouse Hepatitis Virus: Negative Reovirus Screen: Negative Lymphocytic Choriomeningitis virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Mouse Rotavirus: Negative Theiler's Murine Encephalomyelitis: Negative Ectromelia/Mousepox Virus: Negative Hantavirus: Negative Polyoma Virus: Negative Mouse Adenovirus: Negative Sendai Virus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Mouse Cytomegalovirus: Negative K Virus: Negative |
| 保存 | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
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Recommended Isotype Control(s)
RecombiMAb mouse IgG2a isotype control, unknown specificity
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Recommended Dilution Buffer
InVivoPure pH 7.0 Dilution Buffer
